ABSTRACT
Objective:To investigate the role of Notch signaling pathway in the migration of hepatocellular carcinoma cells and its related mechanisms.Methods:HepG2 was cultured in vitro,which was HL-7702,Huh-7.The migration ability of Transwell cells was detected,Notch,Snail,Westernblot to detect the expression of E-cadherin protein.1 mol/L DAPT and 5 mol/L DAPT were used to block the Notch signaling pathway,and compared the effects of different concentrations on cell migration and the expression of Snail and E-cadherin protein in hepatocellular carcinoma cells.Results:The invasion and migration ability of HCC cell lines was significantly higher than that of normal non neoplastic liver cells (P＜0.05).The invasion and migration ability of HepG2 was slightly higher than that of Huh-7,but the difference was not statistically significant (P＞0.05).Used Western blot method to detect protein imprinting cells Notch,SnaiI,E-cadherin protein expression,results showed that the expression of Notch and Snail in liver cancer cells than normal cells,the expression of E-cadherin was significantly lower than that of normal cells.Blocking the Notch signaling pathway by 1 mol/L DAPT and 5 mol/L DAPT results showed that compared with the control group,the expression of 1 mol/L DAPT and 5 mol/L DAPT can significantly reduce Snail,increased the expression of E-cadherin,and with the increase of the concentration of strengthening effect (P＜0.05).Conclusion:Notch signaling pathway plays an important role in the invasion and migration of hepatocellular carcinoma cells.It is believed that the expression of Snail and E-cadherin may be related to the expression.
ABSTRACT
Objective:To investigate the role of Notch signaling pathway in the migration of hepatocellular carcinoma cells and its related mechanisms.Methods:HepG2 was cultured in vitro,which was HL-7702,Huh-7.The migration ability of Transwell cells was detected,Notch,Snail,Westernblot to detect the expression of E-cadherin protein.1 mol/L DAPT and 5 mol/L DAPT were used to block the Notch signaling pathway,and compared the effects of different concentrations on cell migration and the expression of Snail and E-cadherin protein in hepatocellular carcinoma cells.Results:The invasion and migration ability of HCC cell lines was significantly higher than that of normal non neoplastic liver cells (P＜0.05).The invasion and migration ability of HepG2 was slightly higher than that of Huh-7,but the difference was not statistically significant (P＞0.05).Used Western blot method to detect protein imprinting cells Notch,SnaiI,E-cadherin protein expression,results showed that the expression of Notch and Snail in liver cancer cells than normal cells,the expression of E-cadherin was significantly lower than that of normal cells.Blocking the Notch signaling pathway by 1 mol/L DAPT and 5 mol/L DAPT results showed that compared with the control group,the expression of 1 mol/L DAPT and 5 mol/L DAPT can significantly reduce Snail,increased the expression of E-cadherin,and with the increase of the concentration of strengthening effect (P＜0.05).Conclusion:Notch signaling pathway plays an important role in the invasion and migration of hepatocellular carcinoma cells.It is believed that the expression of Snail and E-cadherin may be related to the expression.